We are currently pursuing two lines of research focused on the development of bioengineering practice to enable cell-based treatments in regenerative medicine:  (a) quantitative analysis of protein dynamics within the cell and its environment, and (b) multiphoton-activation of prodrugs in cells and tissues.  The hypothesis driving this research program is that an understanding of transport in the cytoplasm and cellular microenvironment is required for the full realization of host-integrated cellular transplants.  The primary tool  used for this work is multiphoton laser scanning microscopy (MP-LSM).  I am a member of the Canadian Stem Cell National Centers of Excellence.  My contribution to this network is focused on identifying proteins that are important in stem cell differentiation through the application of functional proteomics, and long-term stem cell imaging and tracking for stem cell lineage analysis.